The Boonshoft School of Medicine & The College of Science+Mathematics

Research

Faculty

Topics

Cancer

DNA Replication & Repair

Host-Microbial/Pathogen Interactions

Metabolomics/Metabolism

Nuclear Receptors

Protein Structure Function

Retinal Cell Biology

Riboswitches

Signal Transduction

Techniques

Protein Biochemistry

  • Protein purification
  • SDS-PAGE
  • Western Blotting
  • Ligand binding
  • Stopped Flow
  • Circular Dichroism (CD)
  • Proton Pumping
  • Enzymatic reactions
  • Kinetics
  • Mass spectrometry
  • Fluorescence resonance energy transfer (FRET)
  • In silico protein modeling
  • Protein docking
  • HPLC
  • Liposome characterization
  • Mitochondria isolations

Gene Regulation

  • RNA isolation
  • Northern blotting
  • Plasmid reporter assays
  • qRT-PCR
  • Western blotting
  • RNA interference
  • EMSA / gel shifts
  • Chromatin Immunoprecipitation
  • microRNAs
  • Molecular Cloning
  • Oligonucleotide microarray
  • Next generation sequencing
  • SNP detection

Molecular Genetics

  • Neurospora
  • Yeast
  • Transgenic mice
  • Mutant Screens
  • Animal cell culture
  • Xenopus egg extracts
  • PCR
  • Mutant Mice
  • Fluorescence in situ hybridization (FISH)

Cell/Tissue Imaging

  • Immunofluroescence
  • Live cell imaging
  • Immunohistochemistry
  • Fluorescence resonance energy transfer (FRET)
  • Molecular colocalization
  • Bimolecular fluorescence complementation (BiFC)
  • Fluorescent proteins
  • Cell sorting

Metabolomics

  • Nuclear magnetic resonance (NMR)
  • Mass spectrometry
  • High pressure liquid chromatography (HPLC)
  • Thin layer chromatography (TLC)

Microbiology

  • Aerobic culturing
  • Anaerobic culturing
  • In vitro fermentation
  • Clinical microbiology
  • Phylogenetic analysis
  • Microbial community analysis
  • Microbial identification