The Boonshoft School of Medicine & The College of Science+Mathematics

Research

Faculty

Topics

Cancer

DNA Replication & Repair

Host-Microbial/Pathogen Interactions

Metabolomics/Metabolism

Nuclear Receptors

Protein Structure Fuction

Retinal Cell Biology

Daniel T. Organisciak, Ph.D., Professor
Retinal light damage, cell death and antioxidants

Riboswitches

John V. Paietta, Ph.D., Associate Professor
Riboswitches and gene regulation in Neurospora

Signal Transduction

Techniques

Protein Biochemistry

  • Protein purification
  • SDS-PAGE
  • Western Blotting
  • Ligand binding
  • Stopped Flow
  • Circular Dichroism (CD)
  • Proton Pumping
  • Enzymatic reactions
  • Kinetics
  • Mass spectrometry
  • Fluorescence resonance energy transfer (FRET)
  • In silico protein modeling
  • Protein docking
  • HPLC
  • Liposome characterization
  • Mitochondria isolations

Gene Regulation

  • RNA isolation
  • Northern blotting
  • Plasmid reporter assays
  • qRT-PCR
  • Western blotting
  • RNA interference
  • EMSA / gel shifts
  • Chromatin Immunoprecipitation
  • microRNAs
  • Molecular Cloning
  • Oligonucleotide microarray
  • Next generation sequencing
  • SNP detection

Molecular Genetics

  • Neurospora
  • Yeast
  • Transgenic mice
  • Mutant Screens
  • Animal cell culture
  • Xenopus egg extracts
  • PCR
  • Mutant Mice
  • Fluorescence in situ hybridization (FISH)

Cell/Tissue Imaging

  • Immunofluroescence
  • Live cell imaging
  • Immunohistochemistry
  • Fluorescence resonance energy transfer (FRET)
  • Molecular colocalization
  • Bimolecular fluorescence complementation (BiFC)
  • Fluorescent proteins
  • Cell sorting

Metabolomics

  • Nuclear magnetic resonance (NMR)
  • Mass spectrometry
  • High pressure liquid chromatography (HPLC)
  • Thin layer chromatography (TLC)

Microbiology

  • Aerobic culturing
  • Anaerobic culturing
  • In vitro fermentation
  • Clinical microbiology
  • Phylogenetic analysis
  • Microbial community analysis
  • Microbial identification