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Safe, Green Labs
Did you know that laboratories use 4-5 times more energy than an office space per square foot? Green your lab and share your efforts. Conserve energy and water use, reduce hazardous waste, recycle materials, purchase green, and stay safe.
OSHA's Occupational Exposure to Hazardous Chemicals in Laboratories standard (29 CFR 1910.1450), referred to as the Laboratory standard, covers laboratories where chemical manipulation generally involves small amounts of a limited variety of chemicals. This standard applies to all hazardous chemicals meeting the definition of “laboratory use” and having the potential for worker exposure.
A Laboratory means a facility where the “laboratory use of hazardous chemicals” occurs. Wright State has many locations where relatively small quantities of hazardous chemicals are used on a non-production basis.
Laboratory use of hazardous chemicals means handling or use of such chemicals in which all of the following conditions are met:
- Chemical manipulations are carried out on a “laboratory scale” (i.e., work with substances in which the containers used for reactions, transfers, and other handling of substances is designed to be easily handled by one person);
- Multiple chemical procedures or chemicals are used;
- The procedures involved are not part of a production process, nor do they in any way simulate a production process; and
- "Protective laboratory practices and equipment” are available and in common use to minimize the potential for worker exposure to hazardous chemicals.
Please refer to the electronic copy to ensure you have the current revision.
- Chemical Hazards
- Chemical Hazards
- Chemical Fume Hoods
- Biological Hazards
- Biological Hazards
- Working with Small Animals
- Biosafety Cabinets
- Physical Hazards
- Ergonomics for the Prevention of Musculoskeletal Disorders in Laboratories
- Safety Hazards
- Safety Hazards
- Cryogens and Dry Ice
- Electrical Hazards
- Other Hazards
- Latex Allergies
Environmental Health and Safety (EHS) offers occupational and environmental training to Wright State University employees and students. Training is available in classroom format and online through Pilot.
Enrollment in the Animal Contact Medical Surveillance Program is mandatory for all personnel who have contact with animals. This program is used to identify and to enroll animal users in preventive medicine programs and to provide occupational health information related to the use and care of animals at Wright State University.
The Biological Safety Program provides services related to the use of biological agents. Biological agents include bacteria, viruses, fungi, other microorganisms and their associated toxins. They may adversely affect human health in a variety of ways, ranging from relatively mild, allergic reactions to serious medical conditions, even death.
Types of regulated biological agents include
- human, animal, and plant pathogens;
- human blood and blood components;
- recombinant/synthetic nucleic acids;
- cell and tissue cultures; and
- biological toxins.
Program services include
- oversight of the receipt, possession, use, and transfer of biological materials;
- select agent registration;
- oversight of the University’s Bloodborne Pathogens Program;
- safe laboratory practices;
- Laboratory Biological Safety Level (BSL) assignment;
- safety training;
- waste disposal;
- surveillance and certification of biological safety cabinets; and
- review of recombinant/synthetic nucleic acids applications
Institutional Biosafety Committee (IBC)
The Institutional Biosafety Committee (IBC) is responsible for monitoring and ensuring the compliance of Wright State University laboratories with the NIH Guidelines.
The purpose of the NIH guidelines is to specify appropriate risk-based safety practices and containment measures are employed for:
- recombinant nucleic acid molecules,
- synthetic nucleic acid molecules, including those that are chemically or otherwise modified but can base pair with naturally occurring nucleic acid molecules, and
- cells, organisms, and viruses containing such molecules.
The IBC provides oversight of these recombinant/synthetic nucleic acid molecule research projects, including reviewing and approving of all research projects involving recombinant/synthetic nucleic acid molecules. The IBC responsibilities include:
- conducting comprehensive risk assessments,
- approving IBC Protocols,
- institutes appropriate corrective measures,
- develops policies and procedures, and
- reports to and communicates with NIH
The Biosafety Officer responsibilities include:
- voting member of the IBC,
- reviews all IBC protocols,
- performs laboratory inspections,
- provides emergency and incident response,
- coordinates with regulatory agencies,
- provides biosafety training, and
- consults with campus staff
The Principal Investigator (PI) is responsible for full compliance with the NIH Guidelines in the conduct of recombinant or synthetic nucleic acid molecule research. The NIH Office of Biotechnology Activities (OBA) provides a brochure for PIs to review. The information should be posted in the laboratory.
The Department of Environmental Heath and Safety (EHS) recognizes that manipulation and insertion of recombinant/synthetic nucleic acids into various organisms is an integral part of many of research and teaching laboratories.
For more information, please click on the links below.
Definition of Recombinant and Synthetic Nucleic Acid Molecules
- molecules that a) are constructed by joining nucleic acid molecules and b) that can replicate in a living cell, i.e., recombinant nucleic acids;
- nucleic acid molecules that are chemically or by other means synthesized or amplified, including those that are chemically or otherwise modified but can base pair with naturally occurring nucleic acid molecules, i.e., synthetic nucleic acids, or
- molecules that result from the replication of those described in (i) or (ii) above.
IBC Protocol Applications for Recombinant or Synthetic Nucleic Acids Research
Anyone intending to perform activities involving recombinant DNA or biohazardous agents must submit a protocol to the IBC for consideration, including those activities which are exempt from the NIH Guidelines.
The following steps summarize the procedure for a new IBC protocol submission:
- Complete the New IBC Protocol Application Form (PDF)
- Submit the completed application to the IBSO for review
- IBSO returns reviewed application to IBC applicant
- Applicant prepares copies for Research and Sponsored Programs (RSP)
- 2 copies for Biosafety Level 1 (BSL1)
- 18 copies for Biosafety Level 2 (BSL2) or higher activities
- Applicant submits original IBC application and copies to RSP
- Copies are distributed to IBC members for review
- Protocol is reviewed at monthly meeting
- Applicant is notified of IBC protocol determination
In addition to the Institutional Biosafety Committee (IBC),
- any human subject MUST be reviewed by the Institutional Review Board (IRB);
- any animal use MUST be reviewed by the Institutional Animal Care and Use Committee (IACUC); and
- any use of radioactive materials or radiation generating equipment MUST be reviewed by the Radiation Safety Committee (RSC).
- Marjorie Markopoulos, Institutional Biological Safety Officer (IBSO) 937-775-2797
- Greg Merkle, Environmental Health and Safety Specialist 937-775-2217
- Mandy Karper, IBC Facilitator 937-775-3332
An ideal laboratory safety culture ensures that anyone who enters a laboratory, from inexperienced students to senior investigators, understands that they are entering an environment that requires special precautions. They are aware of the hazards posed by the materials they and others in the lab are working with, and they are prepared to take immediate and appropriate measures to protect themselves and their co-workers, especially in the case of unexpected events. At a minimum laboratory safety includes:
- awareness of the physical and chemical properties and health hazards of laboratory reagents and equipment being used, gained by conducting hazard analysis,
- availability and use of proper apparatus and infrastructure needed to carry out the procedure safely,
- knowledge of and ability to execute any additional special practices necessary to reduce risks, use of proper personal protective equipment,
- access to a well-organized workspace that facilitates unrestricted movement about the laboratory and appropriate segregation of materials and processes, and
- familiarity with emergency procedures, including the use of safety showers, fire extinguishers, and eye stations.
A strong positive safety culture encourages all laboratory workers to place the highest priority on these practices. It is not enough to provide safe equipment, systems, and procedures if the culture of the organization does not encourage and support working safely.
Chemical Compatibility Information Sources
EPA’s Chemical Compatibility Chart (EPA-600/2-80-076 April 1980 A METHOD FOR DETERMINING THE COMPATIBILITY OF CHEMICAL MIXTURES)
- EPA Chemical Compatibility Chart (PDF) gives names of chemical classes with which the chosen class is incompatible. Legend indicates "Consequences" that can be expected when mixing chemical wastes.
Cameo Chemicals: Database of Hazardous Materials Office of Response and Restoration, National Ocean Service, NOAA
- Database of reactivity information for more than 5,000 chemicals and chemical mixtures.
- Includes information about the special hazards of each chemical and about whether a chemical reacts with air, water, or other materials.
- Permits virtual "mixing" of chemicals to find out what dangers could arise from accidental mixing.
- Builds a temporary list of chemicals to predict reactivity online.
Chemical Fume Hoods and Local Exhaust Ventilation
For general information on chemical fume hoods see the Wright State Laboratory Safety Manual, Section III, Fume Hood Inspection and Operating Instructions.
Hood Airflow Performance Checks
Environmental Health and Safety checks airflow performance of all campus fume hoods and subsequently issues reports on the status of the fume hoods in each building. EHS rechecks airflow performance after Facilities Operations and Maintenance Personnel have completed work requests.
Mechanical Malfunction or Repair Requests
Contact Customer Care Center (937-775-4444) to report issues, problems or repair requests related to mechanical aspects of the hood, such as alarms, monitors/controllers, or when the hood appears to not be operating correctly. After Facilities Operations completes the work request, the hood airflow is verified by EHS.
Department of Homeland Security's Chemical Facility Anti-Terrorism Standards (CFATS)
The U.S. Department of Homeland Security's (DHS) Chemical Facility anti-Terrorism Standards (CFATS) Final Rule, published November 20, 2007, imposes comprehensive federal security regulations for high-risk chemical facilities. A risk-based approach is used to screen and to secure chemical facilities as determined by the DHS that "present high levels of security risk." In order to make that determination, CFATS requires facilities in possession at or above the listed Screening Threshold Quantity (STQ) of DHS defined Chemicals of Interest (COI) to complete the Chemical Security Assessment Tool (CSAT) Top-Screen.
Chemical of Interest (COI)—Appendix A
DHS uses Appendix A as part of the CFATS process to identify, assess, and secure high-risk facilities. Colleges and universities are among the many types of facilites that possess COI for legitimate uses, and DHS may determine some to be high risk facilites subject to CFATS.
Over 300 COI that DHS believes may pose significant risks to human life or health if misused by terrorists are listed in Appendix A . The COI are organized by security and vulnerability issues by the following potential uses by a terrorist:
- steal, divert, or otherwise acquired to use as a weapon at another time and place (Theft/Diversion COI); or
- sabotage or contaminate to explode or release in transit (Sabotage COI); or
- release as an explosive or to form a flammable or toxic cloud (Release COI).
A chemical facility is defined in 6 CFR Part 27 as "any establishment that possesses or plans to possess, at any relevant point in time, a quantity of a chemical substance determined by the Secretary to be potentially dangerous or that meets other risk-related criteria identified by the Department."
The DHS recognizes that colleges and universities are composed of many individual building, operations, and areas, only some of which may possess COI. A college or university with a high-risk facility on campus - such as a research complex - may decide, for example to implement appropriate security measure only at the high-risk "facility," as opposed to the entire campus.
The Top-Screen is an online questionnaire that collects basic information about a facility's location and operations and is used by DHS to preliminarily determine if the facility is high risk. Any facility that possesses a COI at or above the applicable screening threshold quantities (STQ) listed in Appendix A of CFATS must complete and submit a Top-Screen to DHS with in 60 calendar days of coming into possession of COI. However, a release COI used in a laboratory under the supervision of a "technically qualified individual" need not be counted toward a facility's STQ.
Wright State Responsibilities
Wright State conducts research in chemistry, medicine, and other fields where chemicals of interest (COIs) may be regularly used, stored, or even produced in:
- chemistry labs
- research facilities,
- pool complexes,
- medical programs, or
- other facilities.
Many of these chemicals have the potential to be deliberately diverted, released, or otherwise misused by terrorists.
Chemical Inventory Management
Inventory management is an essential component to comply with the DHS CFATS regulation. According to CFATS, any facility possessing COI has 60 days to report when the amount of any COI meets or exceeds the Screening Threshold Quantity (STQ).
- Laboratories must update inventories every 60 days to ensure regulatory compliance.
- Laboratories must inform EHS when purchasing any COI.
EHS runs a report on all chemical inventories every 60 days to monitor levels of COIs. These updates assists us in preventing any COI that is close to the STQ from exceeding it or enables us to report to DHS within the 60 day limit any COI that is over the STQ.
Compliance is mandatory. Violation of a compliance order issued by DHS may result in additional orders assessing civil penalties of $25,000 per day per violation and/or requiring the facility to cease operations.
Lab Safety Labels
Labels play an important role to ensure a safe environment. Labels identify the hazard and qualities of the contents in the container.
Labels are used to identify a substance and it is often the initial source of information, presented in a written or graphic forms. It is attached or on the outside face of a container.
Environmental Health and Safety has the following free labels available for chemicals commonly found in laboratories. Download by clicking on the link.
Common laboratory chemicals
Let us know if you need a label for your application.
Transport, Transfers, and Movement of Hazardous Materials
Moving chemicals within a building or between adjacent buildings
Spills and chemical exposure can occur if chemicals are moved from one area to another.
- Do not work alone. Never transport hazardous materials by yourself.
- Moving hours: Package and move Hazardous Chemicals only during normal business hours (Monday-Friday 8 a.m.-4 p.m.) so support staff will be available to help if there is a spill or accident.
- Never move open containers of hazardous materials in elevators. Stairs should only be used if an elevator is not available and only for small containers that can be easily carried by hand.
- Complete Lab Safety Training.
- Moving quantities larger than 4L is discouraged.
- Keep chemicals in their original packing when transporting, if possible.
- Know the hazards of the material and know how to handle a spill of the material.
- Wear appropriate personal protective equipment (PPE). Minimum PPE includes ANSI-approved safety glasses, lab coat or other appropriate lab attire, and closed-toe shoes.
- Containers of hazardous materials or chemicals must be labeled and protected from breakage.
- Hazardous materials must be attended at all times during the transportation process.
- All containers must have secure, tight-fitting lids.
- Incompatible chemicals should not be packed together.
- Use a bottle carrier, cart or other secondary container when transporting chemicals in breakable containers through hallways or between buildings. Secondary containers must be
- compatible with the chemical and
- large enough to hold the entire contents being transferred in the event of breakage.
Guidelines and Registration
Laser Eye Examination
Medical evaluation (or documentation of refusal) is required for all Class 3b and 4 laser users in the university and is the responsibility of the Principal Investigator. The examination is administered by an optometrist and is scheduled through the Department Of Environmental Health and Safety (EHS). An appointment for a laser eye exam can be made by calling EHS at (937) 775-2215.
This evaluation must be completed prior to laser use, and shall be performed following suspected laser injury; periodic examinations are not required.
If you don't wish to have the examination please complete the Statement Declining Pre-Placement Eye Exam Form (PDF) to obtain the waiver form which should be printed out and sent to Environmental Health and Safety at firstname.lastname@example.org or deliver to our office at 047 Biological Sciences II.
Nanotechnology is generally defined as the manipulation of engineered structures, devices, and system on a near-atomic scale to produce new structures, materials and devices.
Nanomaterials are defined as those things that have a length scale between 1 and 100 nanometers. At this size, materials begin to exhibit unique properties that affect physical, chemical, and biological behavior.
Basics of Nanomaterial Safety
- Follow all posted laboratory requirements regarding required personal protective equipment
- Obtain training prior to working with nanomaterials and follow instructions
- Do not eat or drink in the laboratory
- Follow good housekeeping procedures.
- Clean all potentially contaminated working surfaces when visibly contaminated and/or at least once a day.
- Use wet wiping or a HEPA vacuum on dry materials
- Wash hands before eating, smoking, or leaving the worksite
- Keep laboratory doors closed and limit access
- This document contains recommendations on engineering controls and safe practices for handling engineered nanomaterials in laboratories and some pilot scale operations. This guidance was designed to be used in tandem with well-established practices and the laboratory’s chemical hygiene plan.
Programs and Policies
Instructions to Submit Forms:
- Click to open and/or save the form
- Complete required information
- Print form
- Sign in Ink and submit to appropriate personnel
Radiation Generating Equipment Forms
Radioactive Materials Forms
Ohio Department of Health (ODH)
The following Ohio Department of Health Rules apply to Wright State's radiation safety program: