B number Gene Name Subunit mRNA intensity mRNA present # of tryptic peptides predicted Protein detected - total list Protein detected - long list Proteins detected - membrane sample Membrane protein assignment Gene Description b0066 yabJ ABC 458 P 12 putative ATP-binding component of a transport system b0067 yabK membrane 774 P 14 GenProt-PHD putative transport system permease protein b0068 tbpA binding protein 957 P 13 P P thiamin-binding periplasmic protein b0151 fhuC ABC 1066 P 14 ATP-binding component of hydroxymate-dependent iron transport b0152 fhuD binding protein 378 P 9 MP-PHD hydroxamate-dependent iron uptake, cytoplasmic membrane component b0153 fhuB membrane 420 P 14 MP-GenProt-PHD hydroxamate-dependent iron uptake, cytoplasmic membrane component b0197 yaeC binding protein 16241 P 13 P P(m) putative lipoprotein b0198 yaeE membrane 3318 P 6 P P(m) GenProt-PHD putative transport system permease protein b0199 abc ABC 1966 P 13 P P(m) ATP-binding component of a transporter b0655 gltI binding protein 5452 P 14 P P glutamate/aspartate transport system periplasmic binding transport protein b0809 glnQ ABC 3281 P 6 P P(m) ATP-binding component of glutamine high-affinity transport system b0810 glnP membrane 4090 P 12 P P(m) MP-GenProt-PHD glutamine high-affinity transport system; membrane component b0811 glnH binding protein 16120 P 14 P P periplasmic glutamine-binding protein; permease b0860 artJ binding protein 4456 P 12 P P arginine 3rd transport system periplasmic binding protein b0861 artM membrane 1059 P 7 GenProt-PHD arginine 3rd transport system permease protein b0862 artQ membrane 1447 P 5 GenProt-PHD arginine 3rd transport system permease protein b0864 artP ABC 4200 P 5 ATP-binding component of 3rd arginine transport system b0886 cydC membrane 665 P 20 GenProt-PHD ATP-binding component of cytochrome-related transport b0887 cydD membrane 380 P 23 P P(m) GenProt-PHD ATP-binding component of cytochrome-related transport, Zn sensitive b1123 potD binding protein 5107 P 14 P P spermidine/putrescine periplasmic transport protein b1124 potC membrane 3389 P 2 GenProt-PHD spermidine/putrescine transport system permease b1125 potB membrane 698 P 7 GenProt-PHD spermidine/putrescine transport system permease b1126 potA ABC 1171 P 19 ATP-binding component of spermidine/putrescine transport b1243 oppA binding protein 7098 P 26 P P oligopeptide transport; periplasmic binding protein b1244 oppB membrane 3242 P 9 P P(m) GenProt-PHD oligopeptide transport permease protein b1245 oppC membrane 2301 P 5 GenProt-PHD homolog of Salmonella oligopeptide transport permease protein b1246 oppD ABC 1533 P 20 homolog of Salmonella ATP-binding protein of oligopeptide ABC transport system b1290 sapF ABC 516 P 15 putative ATP-binding protein of peptide transport system b1291 sapD ABC 569 P 19 putative ATP-binding protein of peptide transport system b1292 sapC membrane 427 P 4 GenProt-PHD homolog of Salmonella peptide transport permease protein b1293 sapB membrane 141 A 8 GenProt-PHD homolog of Salmonella peptide transport permease protein b1294 sapA binding protein 763 P 25 P P homolog of Salmonella peptide transport periplasmic protein b1709 btuD ABC 1013 P 9 ATP-binding component of vitamin B12 transport system b1711 btuC membrane 334 A 6 GenProt-PHD vitamin B12 transport permease protein b1857 yebL binding protein 1559 P 14 P P PHD putative adhesin b1917 yecC ABC 912 P 8 P P(m) putative ATP-binding component of a transport system b1918 yecS membrane 1087 P 10 GenProt-PHD putative transport system permease protein (former yecC) b1920 fliY binding protein 5516 P 18 P P putative periplasmic binding transport protein b2148 mglC membrane 543 P 6 P P(m) GenProt-PHD methyl-galactoside transport and galactose taxis b2149 mglA ABC 1844 P 33 ATP-binding component of methyl-galactoside transport and galactose taxis b2150 mglB binding protein 7569 P 20 P P galactose-binding transport protein; receptor for galactose taxis b2306 hisP ABC 974 P 9 P P(m) ATP-binding component of histidine transport b2307 hisM membrane 1121 P 9 MP-GenProt-PHD histidine transport, membrane protein M b2308 hisQ membrane 1082 P 6 GenProt-PHD histidine transport system permease protein b2310 argT binding protein 3551 P 15 P P lysine-, arginine-, ornithine-binding periplasmic protein b2422 cysA ABC 2069 P 19 P P(m) ATP-binding component of sulfate permease A protein; chromate resistance b2423 cysW membrane 1423 P 6 GenProt-PHD sulfate transport system permease W protein b2425 cysP binding protein 3266 P 21 P P thiosulfate binding protein b2677 proV ABC 4860 P 13 P P(m) ATP-binding component of transport system for glycine, betaine and proline b2678 proW membrane 5333 P 3 P P P(m) GenProt-PHD high-affinity transport system for glycine betaine and proline b2679 proX binding protein 6568 P 11 P P high-affinity transport system for glycine betaine and proline b3450 ugpC ABC 769 P 9 P P(m) ATP-binding component of sn-glycerol 3-phosphate transport system b3451 ugpE membrane 557 P 6 MP-GenProt-PHD sn-glycerol 3-phosphate transport system, integral membrane protein b3452 ugpA membrane 470 P 4 MP-GenProt-PHD sn-glycerol 3-phosphate transport system, integral membrane protein b3453 ugpB binding protein 760 P 25 P P sn-glycerol 3-phosphate transport system; periplasmic binding protein b3454 livF ABC 4687 P 11 P P(m) ATP-binding component of leucine transport b3455 livG ABC 3665 P 15 P P P(m) ATP-binding component of high-affinity branched-chain amino acid transport system b3456 livM membrane 2830 P 9 GenProt-PHD high-affinity branched-chain amino acid transport b3457 livH membrane 3491 P 5 P P(m) MP-GenProt-PHD high-affinity branched-chain amino acid transport system; membrane component b3460 livJ binding protein 26232 P 13 P P P(m) high-affinity amino acid transport system; periplasmic binding protein b3540 dppF ABC 1835 P 16 P P(m) putative ATP-binding component of dipeptide transport system b3541 dppD ABC 3918 P 17 P P(m) putative ATP-binding component of dipeptide transport system b3542 dppC membrane 2028 P 5 GenProt-PHD dipeptide transport system permease protein 2 b3543 dppB membrane 2021 P 6 P P(m) GenProt-PHD dipeptide transport system permease protein 1 b3544 dppA binding protein 13089 P 24 P P P(m) dipeptide transport protein b3566 xylF binding protein 247 P 13 P P xylose binding protein transport system b3567 xylG ABC 141 P 28 putative ATP-binding protein of xylose transport system b3568 xylH membrane 144 A 10 MP-GenProt-PHD putative xylose transport, membrane component b3749 rbsA ABC 479 P 30 ATP-binding component of D-ribose high-affinity transport system b3750 rbsC membrane 1311 P 8 GenProt-PHD D-ribose high-affinity transport system b3751 rbsB binding protein 11090 P 13 P P D-ribose periplasmic binding protein b4032 malG membrane 535 P 8 MP-GenProt-PHD part of maltose permease, inner membrane b4033 malF membrane 630 P 14 GenProt-PHD part of maltose permease, periplasmic b4034 malE binding protein 3061 P 24 P P periplasmic maltose-binding protein; substrate recognition for transport and chemotaxis b4035 malK ABC 1416 P 17 ATP-binding component of transport system for maltose The list of known E. coli ABC-type transporters was taken from http://66.93.129.133/transporter/wb/index2.html (Dassa et al., 1999). This list was matched against the list of E. coli proteins detected by HPLC-MS/MS (Corbin et al., 2003). Annotation information was taken from the E.coli Entry Point database. The annotation in the database is based on Blattner et al (1997). Functional categories are assigned as defined by Riley and Labedan (1996). Subunit assignment was taken from http://66.93.129.133/transporter/wb/index2.html. Membrane protein assignment (see Corbin et al.): MP - list # 1, GenProt - list #2, PHD - list #3.