R.W. Corbin*, O. Paliy*, F. Yang, J. Shabanowitz, M. Platt, C.E. Lyons, K. Root, J. McAuliffe, M.I. Jordan, S. Kustu, E. Soupene, and D.F. Hunt [* equal contribution]

Toward a protein profile of Escherichia coli: comparison to its transcription profile. (2003) Proc. Natl. Acad. Sci. USA, 100(16), 9232-9237.

Full text (pdf)

Supplementary material:

• Protocol for sample preparation for HPLC-MS/MS


• Protocol for preparation, labelling, and hybridization of samples to GeneChips


• Protein detection limit (pdf)



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• Complete table of all E. coli genes with mRNA and protein data (pdf)


• Complete table of all genes in the expanded short list (pdf)


• Complete table of all genes in the expanded total list (pdf)


• Table: protein length as a function of protein functional category (pdf)



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• Chart 1: protein length as a function of mRNA intensity (pdf)


• Chart 2: number of predicted tryptic peptides per protein as a function of mRNA intensity (pdf)