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Diggs Laboratory for Life Science Research : Department of Biochemistry and Molecular Biology : Wright State University
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R.W. Corbin*, O. Paliy*, F. Yang, J. Shabanowitz, M. Platt, C.E. Lyons, K. Root, J. McAuliffe, M.I. Jordan, S. Kustu, E. Soupene, and D.F. Hunt [* equal contribution]

Toward a protein profile of Escherichia coli: comparison to its transcription profile. (2003) Proc. Natl. Acad. Sci. USA, 100(16), 9232-9237.


Full text (pdf)

Supplementary material:


  • Protocol for sample preparation for HPLC-MS/MS

  • Protocol for preparation, labelling, and hybridization of samples to GeneChips

  • Protein detection limit (pdf)



  • Complete table of all E. coli genes with mRNA and protein data (pdf)

  • Complete table of all genes in the expanded short list (pdf)

  • Complete table of all genes in the expanded total list (pdf)

  • Table: protein length as a function of protein functional category (pdf)



  • Chart 1: protein length as a function of mRNA intensity (pdf)

  • Chart 2: number of predicted tryptic peptides per protein as a function of mRNA intensity (pdf)

 
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