Paliy Lab
Diggs Laboratory for Life Science Research : Department of Biochemistry and Molecular Biology : Wright State University
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O. Paliy and B. Foy

Mathematical modeling of 16S ribosomal DNA amplification reveals optimal conditions for the interrogation of complex microbial communities with phylogenetic microarrays (2011) Bioinformatics, 27(15): 2134-40.


Full text (pdf)


Supplementary material:


  • Supplementary figure S1 (png): Agarose gel electrophoresis of gDNA samples amplified with different number of PCR cycles

  • Supplementary figure S2 (png): Simulation results - average sample amplification rate vs PCR cycle number

  • Supplementary figure S3 (png): Simulation results - fraction of 16S rDNA in amplified sample vs PCR cycle number

  • Supplementary figure S4 (png): Simulation results - deviation of relative 16S rDNA abundances with PCR cycle number [graphs are shown for most abundant gDNA species in the original sample {rank 1}, mid-abundant {rank 200}, and least abundant {rank 400}]

  • Supplementary figure S5 (png): Simulation results - yield of PCR reaction as a function of PCR cycle number and amount of starting gDNA [no loss due to PCR reaction purification is assumed]

  • Supplementary figure S6 (png): Simulation results - number of PCR-amplified samples needed to becombined to obtain 1500 bp microarray load [we assume 50% loss of DNA during PCR purification]

  • Supplementary table S1 (pdf): Comparison of simulated and experimental results

  • Detailed model description (pdf)

  • Matlab files used to run simulation (zip)


 
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