Paliy Lab
Diggs Laboratory for Life Science Research : Department of Biochemistry and Molecular Biology : Wright State University
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E. Soupene, W.C. van Heeswijk, J. Plumbridge, V. Stewart, D. Bertenthal, H. Lee, G. Prasad, O. Paliy, P. Charernnoppakul, and S. Kustu

Physiological studies of Escherichia coli strain MG1655: growth defects and apparent cross-regulation of gene expression. (2003) J. Bacteriol., 185(18), 5611-5626.


Full text (pdf)

Comparison of E. coli strains using glass slide and Affymetrix microarrays - expanded section (pdf)


Supplementary material:


  • Protocol for preparation, labeling, and hybridization of samples to GeneChips

  • CGSC6300(MG1655)-NCM3722 (WT) comparison on Affymetrix E. coli Antisense GeneChips and glass slide DNA microarrays

    • All data (tab-delimited txt file, export to Excel or another appropriate program to align)

    • Operons mentioned in the paper (pdf)

  • Glutamine-ammonium comparison for CGSC6300 (MG1655) and E. coli urinary tract isolates on glass slide DNA microarrays

    • All data (tab-delimited txt file, export to Excel or another appropriate program to align)

    • Genes of NtrC regulon (pdf)

    • Genes overexpressed on glutamine in all strains (pdf)

    • Genes overexpressed on ammonium chloride in all strains (pdf)

    • Genes overexpressed on glutamine in CGSC6300 only (pdf)

    • Genes overexpressed on ammonium chloride in CGSC6300 only (pdf)

  • Ethanolamine-ammonium comparison for NCM3722 (WT) and NCM3954 (eut+) on glass slide DNA microarrays: genes overexpressed on ethanolamine (pdf)

 
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