Paliy Lab
Diggs Laboratory for Life Science Research : Department of Biochemistry and Molecular Biology : Wright State University
WSU_logo

L. Rigsbee, R. Agans, B. Foy, and O. Paliy

Optimizing the analysis of human intestinal microbiota with phylogenetic microarray (2011) FEMS Microbiol. Ecol., 75:332-42.


Full text (pdf)

Supplementary material:


  • Supplementary table S1 (pdf): Abundance of bacterial classes as a function of the number of PCR amplification cycles

  • Supplementary table S2 (pdf): Abundance of bacterial genera as a function of the number of PCR amplification cycles

  • Supplementary table S3 (pdf): Number of 16S rRNA genes per bacterial genome - genus-level averages

  • Supplementary table S4 (pdf): Comparison of Microbiota Array data with and without adjustment algorithms use to previous FISH studies

  • Supplementary figure S1 (png): PCA analysis of gDNA samples profiled on Microbiota Array

  • Supplementary figure S2 (png): Distribution of genus-level PCR bias

  • Supplementary figure S3 (png): RNA vs DNA abundance: activity index comparison between two samples

  • Supplementary figure S4 (png): Genus-wide number of 16S sequences of fecal intestinal microbiota vs median number of 16S rRNA gene copies per genome

  • Supplementary figure S5 (png): RNA vs DNA abundance: comparison for individual samples

  • List of qPCR primers used (pdf)

  • Explanation of the principle of CH adjustment algorithm calculation (pdf)

 
Return to:
Dr. Paliy faculty profile
Department of Biochemistry and Molecular Biology
School of Medicine
Wright State University
Visitors:
Friday, 19-Sep-2014 22:44:59 EDT
Site created and maintained by Dr. Oleg Paliy : Developed for Mozilla Firefox : Last updated 02-Jun-14