L. Rigsbee, R. Agans, B. Foy, and O. Paliy
Optimizing the analysis of human intestinal microbiota with phylogenetic microarray (2011) FEMS Microbiol. Ecol., 75:332-42.
Full text (pdf)
Supplementary material:
- Supplementary table S1 (pdf): Abundance of bacterial classes as a function of the number of PCR amplification cycles
- Supplementary table S2 (pdf): Abundance of bacterial genera as a function of the number of PCR amplification cycles
- Supplementary table S3 (pdf): Number of 16S rRNA genes per bacterial genome - genus-level averages
- Supplementary table S4 (pdf): Comparison of Microbiota Array data with and without adjustment algorithms use to previous FISH studies
- Supplementary figure S1 (png): PCA analysis of gDNA samples profiled on Microbiota Array
- Supplementary figure S2 (png): Distribution of genus-level PCR bias
- Supplementary figure S3 (png): RNA vs DNA abundance: activity index comparison between two samples
- Supplementary figure S4 (png): Genus-wide number of 16S sequences of fecal intestinal microbiota vs median number of 16S rRNA gene copies per genome
- Supplementary figure S5 (png): RNA vs DNA abundance: comparison for individual samples
- List of qPCR primers used (pdf)
- Explanation of the principle of CH adjustment algorithm calculation (pdf)
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