O. Paliy, H. Kenche, F. Abernathy, and S. Michail

High-throughput quantitative analysis of human intestinal microbiota with phylogenetic microarray (2009) [in review]

Full text (pdf)

Supplementary material:

• Expanded figure 1 with individual data points plotted (pdf)


• Properties of microbiota array:
  
  
  
  • Number of GC nucleotides per probe: array-wide histogram distribution (image). Probe GC distributions for Affymetrix E. coli, B. subtilis, and Neurospora arrays are shown for comparison; image provided by Affymetrix, Inc.
  
  
  • List of intestinal microbiota phylo-species that can be detected by the array (pdf); phylogenetic classification for each phylo-species is included
• Additional datasets:
  
  
  
  • Identification of microbiota array detection limit for different types of hybridized sample: raw microarray data (pdf)
  
  
  • Analysis of human fecal samples: normalized microarray data (pdf). The data was normalized using MAS5-Loess-MedianPolish algorithm workflow. Data represents non-transformed signal values for each probeset ("Signal") and GCOS-based statistical estimate of probeset detection in each sample ("Call").
• qPCR primers and mathematical calculations employed (pdf)