Dr. Don Cipollini
Professor, Department of Biological Sciences
Director, Environmental Sciences PhD Program
3640 Colonel Glenn Hwy.
Phone (937) 775-3805
FAX (937) 775-3320 ††
Work in my laboratory generally focuses on the chemical ecology of plant responses to the environment, with a focus on induced plant responses to herbivores and pathogens and the chemical ecology of invasive plants, insects, and microbes. One important theme is the influence of environmental factors on the expression of phenotypic plasticity and the outcomes of interactions of plants with their biotic environment.† Within this context, my interests range from highly mechanistic explanations of induced plant responses and their impacts on herbivores and plant fitness, to evolution of plant defenses, to community level impacts. The current project areas described below represent the major thrusts of our research program, although specific directions are often driven by student interests and the availability of funding. In particular, you will see that we have adopted Arabidopsis thaliana as an ecological model and have extended our understanding of plant resistance to understand invasiveness and impacts of Alliaria petiolata, an important invasive plant. †Our studies have extended to the expression and role of constitutive and inducible defenses in woody plant ecology, including that of the invasive shrub, Lonicera maackii.† We have worked experimentally with bacteria, fungi, insects, and plants, and use a variety of techniques including basic experimental greenhouse and field ecology techniques, microscopy, molecular analyses, and analytical chemistry techniques such as HPLC.
ecology of the invasive plants, garlic mustard
(Alliaria petiolata) and Amur honeysuckle (Lonicera maackii)
Garlic mustard is a Eurasian biennial herb that was first noted in North America in the mid 1800ís.† Since its introduction, this plant has spread throughout
Current projects include:
●Experimental tests of purported invasive mechanisms
●Sources of variation and novelty in the expression of chemical defenses
●Growth and defense responses to insect damage, powdery mildew infection, and their interaction
●Identity and impacts of allelochemicals on plants and their associated mycorrhizae
●Assessment of impacts on plant and animal species and genetic diversity of invaded communities
●Chemical defense responses to climate change
and benefits of induced plant responses to insects
and pathogens in Arabidopsis thaliana
Interest in this system lies primarily in costs, benefits, and tradeoffs of plant responses mediated by salicylic acid and jasmonic acid, which we commonly use in conjunction with genetic mutants to manipulate and study plant defense responses at the biochemical, physiological, and ecological level.† Interest also lies in integrated plant responses to herbivores, light, competitors, and other ecological stimuli.† We have also commonly used Arabidopsis as a target plant in allelopathy studies.† Various aspects of our work on Arabidopsis have been funded by USDA, the Ohio Plant Biotechnology Consortium, the Eppley Foundation for Research, and Sigma Xi.
Current projects include:
●Physiological and ecological costs of resistance
●Interactions between plastic responses to herbivores and competitors
●Functional ecology of methyl jasmonate
●Exploring the potential of Plant Growth Promoting
Rhizobacteria to enhance growth and insect resistance
Chemical ecology of induced resistance in woody plants
Our interest in inducible chemical defenses has
extended to woody plants, including Austrian Pine and Ash species, in
collaboration with Dan Herms and
Current Projects Include:
●Chemical ecology of Austrian Pine responses to insects and pathogens across a nutrient gradient
●Mechanisms and variation in ash resistance to Emerald Ash Borer
Conservation and restoration ecology
My wife, Kendra Cipollini,
and I have collaborated on a number of things, including producing our sons,
Emmett and Otto, and in her work on the federally endangered wetland plant, Scirpus ancistrochaetus.
Kendra formerly worked as a conservation scientist with the Nature Conservancy
and is a now an assistant professor in Ecology at
Current projects include:
●Impacts of deer browsing and method of control on restoration success in honeysuckle-remediated areas.
●Interactive effects of water level and nutrient availability on competitive associations of Scirpus ancistrochaetus and Scirpus cyperinus
●Population status and genetic diversity of Scirpus ancistrochaetus,
a federally endangered wetland sedge
Curriculum Vitae and Publications
Tropical Field Ecology (OTS 94-1), Organization for
Professor, (2008-present), Dept. of Biological Sciences, Wright State University
Director (2009-present), Environmental Sciences PhD Program, Wright State University
Interim Director, (2007-2009) Environmental Sciences PhD Program, Wright State University
Associate Professor, (2004-2008), Dept. of Biological Sciences, Wright State University
Assistant Professor, (1999-2004), Dept. of Biological Sciences, Wright State University
Postdoctoral Research Associate, (1997-1999), University of Chicago
Graduate Teaching and Research Assistant, (1992-1997),
Graduate Teaching and Research Assistant, (1990-1992),
†††††††††† 60.† Cipollini, D., Wang, Q., Whitehill, J.G.A., Powell, J., Bonello, P, and Herms, D. 2011.
Distinguishing defensive characteristics in the phloem of ash species resistant and susceptible to the Emerald Ash Borer.† Journal of Chemical Ecology (in press)
59.† Enright, S., and Cipollini, D.† 2011.† Overlapping defense responses to water limitation and
Pathogen attack and their consequences for resistance to powdery mildew disease in garlic mustard, Alliaria petiolata.† Chemoecology† 21: 89-98.† DOI: 10.1007/s00049-011-0072-8
55.† Ciola, V., and Cipollini, D. 2011.† Distribution and host range of a powdery mildew fungus
attacking garlic mustard in southwestern Ohio.† American Midland Naturalist (in press)
54.† Cipollini, K. and Cipollini, D.† 2011.† Population status and threats to the federally endangered
Scirpus ancistrochaetus: implications for management.† Northeastern Naturalist (in press)
†††††††††††††† 53.† Barto, E.K., Powell, J., and Cipollini, D.† 2010.† How novel are the chemical weapons of garlic
mustard in North American forest understories?† Biological Invasions 12: 3465-3471, doi: 10.1007/s10530-010-9744-5
†††††††††††††† 52.† Barto, E.K., Friese, C., and Cipollini, D.† 2010.† Arbuscular mycorrhizal fungi protect a native
plant from allelopathic effects of an invader.† Journal of Chemical Ecology 36: 351-360.
†††††††††††††† 51.† Cipollini, D., and Heil, M.† 2010.† Costs and benefits of induced resistance to pathogens and
herbivores in plants.† CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources doi: 10.1079/PAVSNNR20105005
††††††††††† 50.† Wallis, C., Eyles, A., Chorbadjian R.A., Riedl K., Schwartz S., Hansen R., Cipollini, D., Herms,
††††††††††† D.A., and Bonello P.† 2010.† Differential effects of nutrient availability on the secondary
metabolism of Austrian pine (Pinus nigra) phloem and resistance to Diplodia pinea.† Forest Pathology doi: 10.1111/j.1439-0329.2009.00636.x
49.† Cipollini, D. 2010.† Constitutive expression of methyl jasmonate-inducible responses delays reproduction
†††††††††††††† and constrains fitness responses to nutrients in Arabidopsis thaliana.† Evolutionary Ecology 24: 59-68.
†††††††††††††† 48.† Barto, E.K., and D. Cipollini.† 2009.† Garlic mustard (Alliaria petiolata) removal method affects
†††††††††††††† native establishment.† †Invasive Plant Science and Management 2:230-236.
†††††††††††††† 47.† Barto, E.K., and D. Cipollini.† 2009.† Density dependent phytotoxicity of Impatiens pallida
†††††††††††††† plants exposed to extracts of Alliaria petiolata.† Journal of Chemical Ecology 35: 495-
††††††††† †††† 46.† Cipollini, D., and S. Enright.† 2009.† A powdery mildew fungus levels the playing field for
†††††††††† invasive garlic mustard (Alliaria petiolata) and a North American native plant.† Invasive Plant Science and
†††††††††† Management 2:253-259
45.† Barto, E.K., and D. Cipollini.† 2009.† Field concentrations and half-lives of Alliaria petiolata
secondary metabolites. Chemosphere 76: 71-75
44.† Cipollini, K.A.,
E. Ames, and D. Cipollini.† 2009.† Amur Honeysuckle (Lonicera maackii)
management method impacts restoration of understory plants in the presence of White-Tailed Deer
(Odocoileus virginiana).† Invasive Plant Science and Management 2:45-54
43.† Barto, K., S. Enright, A. Eyles, C. Wallis, R. Chorbadjian, R. Hansen, D.A. Herms, P. Bonello, and D. Cipollini.† 2008.† Effects of fertilization and fungal and insect attack on systemic protein defenses of Austrian pine. Journal of Chemical Ecology 34: 1392-1400
42. Stireman, J.O., and D. Cipollini.† 2008.† Stealth tactics of galling parasites and their potential indirect effects. †New Phytologist, 178: 462-465
40.† Cipollini, D. and M. Dorning.† 2008.† Direct and indirect effects of conditioned soils and tissue extracts of the invasive shrub, Lonicera maackii, on target plant performance. Castanea,
39.† Cipollini, D., R. Stevenson, S. Enright, A. Eyles, P. Bonello.† 2008.† Phenolic metabolites in leaves of the invasive shrub, Lonicera maackii, and their potential phytotoxic and anti herbivore effects. †Journal of Chemical Ecology †34: 144-152
38. Cipollini, K.A., G.Y. McClain, and D. Cipollini.† 2008.†
Separating effects of allelopathy and shading
by Alliaria petiolata and Lonicera maackii on growth, reproduction and survival of Impatiens capensis. †American
37.† Cipollini, D., R. Stevenson, K. Cipollini.† 2008.† Contrasting direct and indirect effects of
allelochemicals from two invasive plants on the performance of a non-mycorrhizal plant. †International Journal of Plant Sciences 169: 371-375
36. †Wallis, C., A. Eyles, R. Chorbadjian, B. McSpadden-Gardener, R. Hansen, D. Cipollini, D. A. Herms, and P. Bonello.† 2008.† Systemic induction of phloem secondary metabolism and induction of resistance to a canker pathogen in Austrian pine. †New Phytologist, 177: 767-778.
35.† Callaway, R.M., D. Cipollini,
K. Barto, G. C. Thelen, S.
G. Hallett, D. Prati, K.
Stinson, J. Klironomos.† 2008.†
Novel weapons: invasive plant suppresses fungal mutualisms in
34. †Eyles, A., D.A. Herms, W. Jones, K. Riedl,
D. Cipollini, S. Schwartz, K. Chan, P. Bonello. 2007.† Comparative
phloem chemistry of Manchurian (Fraxinus
mandshurica) and two North
American ash species (F.
33.† Enright, S. and D. Cipollini.† 2007.† Infection by powdery mildew Erysiphe cruciferarum
(Erysiphaceae) strongly affects growth and fitness of garlic mustard Alliaria petiolata (Brassicaceae). †American Journal of Botany, 94: 1813-1820
32.† Eyles, A., R. Chorbadjian, C. Wallis, R. Hansen, D. Cipollini, D. Herms, P. Bonello.† 2007.† Cross-induction of systemic induced resistance between an insect and a fungal pathogen in Austrian pine over a fertility gradient.† Oecologia, 153: 365-374
31.† Cipollini, D. and B. Gruner.† 2007.† Cyanide in the chemical arsenal of garlic mustard, Alliaria petiolata. †Journal of Chemical Ecology 33: 85-94 pdf
30. †Cipollini, D.† 2007.† Consequences of the overproduction of methyl jasmonate on seed production, tolerance to defoliation, and competitive effect and response of Arabidopsis thaliana. †New Phytologist 173: 146-153† pdf
29. Dorning, M, and D. Cipollini.† 2006.† Leaf and root extracts of the invasive shrub Lonicera maackii inhibit seed germination of three herbs with no autotoxic effects.† Plant Ecology 184: 287-296 pdf
28.† Cipollini, D.† 2005.† Interactive effects of lateral shading and jasmonic acid on morphology, phenology, seed production, and chemical defense responses in Arabidopsis thaliana.† International Journal of Plant Sciences 166: 955-959 pdf
27. Barto, K, and D.F. Cipollini. 2005. Testing predictions of the Optimal Defense Theory and Growth Differentiation Balance Hypothesis in Arabidopsis thaliana. Oecologia 146: 169-178† doi: 10.1007/s00442-005-0207-0 pdf
26. Cipollini, D., J. Mbagwu, K. Barto, C. Hillstrom, and S. Enright. 2005. Expression of constitutive and inducible chemical defenses in native and invasive populations of garlic mustard (Alliaria petiolata). Journal of Chemical Ecology 31: 1243-1255 pdf
25. R.O. Musser, D. F. Cipollini, S.M. Hum-Musser, S.A. Williams, J.K. Brown, and G.W. Felton. 2005. Evidence that the caterpillar salivary enzyme glucose oxidase provides herbivore offense in Solanaceous plants. Archives of Insect Biochemistry and Physiology 58: 128-137.
24. Cipollini, D.F., S. Enright, B. Traw, and J. Bergelson. 2004. Salicylic acid inhibits jasmonic acid-induced resistance of Arabidopsis thaliana to Spodoptera exigua. Molecular Ecology 13: 1643-1653 pdf
23. Cipollini, D.F. 2004. Stretching the limits of plasticity: can a plant defend itself from both competitors and herbivores? Ecology 85: 28-37 (Special feature on Phytohormonal Ecology) pdf
22. Traw, M.B., Kim, J., Enright, S., Cipollini, D.F., and J. Bergelson. 2003. Negative cross talk between salicylate and jasmonate-mediated pathways in the Wassilewskija ecotype of Arabidopsis thaliana. Molecular Ecology 12: 1125-1135 pdf
21. Cipollini, D.F., J.W. Busch, K. Stowe, E. Simms, and J. Bergelson. 2003. Genetic variation and relationships of constitutive and herbivore-induced glucosinolates, trypsin inhibitors, and herbivore resistance in Brassica rapa. Journal of Chemical Ecology 29: 285-302. pdf
20. Cipollini, D.F., C. B. Purrington, and J. Bergelson. 2003. Costs of induced responses. Basic and Applied Ecology 4: 79-89 pdf
19. Cipollini, D.F. 2002. Variation in the expression of chemical defenses in Alliaria petiolata in the field and common garden. American Journal of Botany 89: 1422-1430 pdf
18. Cipollini, M., E. Paulk, and D. Cipollini. 2002. Effect of nitrogen and water treatment on leaf chemistry in horsenettle (Solanum carolinense) and relationship to herbivory by flea beetles (Epitrix spp.) and tobacco hornworm (Manduca sexta) Journal of Chemical Ecology 28: 2377-2398. pdf
17. Cipollini, D.F. 2002. Does competition magnify the fitness costs of induced responses in Arabidopsis thaliana? A manipulative approach. Oecologia 131: 514-520. pdf
16. Cipollini, D.F., and J. Bergelson. 2002. Interspecific competition affects growth and herbivore damage of Brassica napus L. in the field. Plant Ecology 162: 227-231
15. Cipollini, D.F., and M. Sipe. 2001 Jasmonic acid treatment and mammalian herbivory differentially affect chemical defenses and growth of Brassica kaber. Chemoecology 11: 137-143.
14. Cipollini, D.F., and J. Bergelson. 2001. Plant density and nutrient availability constrain the constitutive and wound-induced production of trypsin inhibitors in Brassica napus L. Journal of Chemical Ecology 27: 593-610.† pdf
13. Redman, A, Cipollini, D.F., and J.C. Schultz. 2001. Fitness costs of jasmonic acid-induced defense in tomato, Lycopersicon esculentum. Oecologia 126: 380-385. pdf
12. Cipollini, D.F., and J. Bergelson. 2000. Environmental and developmental regulation of trypsin inhibitor activity in Brassica napus L. Journal of Chemical Ecology 26: 1411-1422. pdf
11. Cipollini, D.F. and J.C. Schultz. 1999. Exploring cost constraints on stem elongation in plants using phenotypic manipulation. American Naturalist 153: 153: 236-242. pdf
10. Cipollini, D.F. 1999. Costs to flowering of the production of a mechanically hardened phenotype in Brassica napus L. International Journal of Plant Sciences 160: 735-741. pdf
9. Cipollini, D.F., and A.M. Redman. 1999. Age-dependent effects of jasmonic acid treatment and wind exposure on foliar oxidase activity and insect resistance in tomato. Journal of Chemical Ecology 25: 271-281 pdf
Lentz, K., and D.F. Cipollini. 1998.
Effect of light and simulated herbivory on growth of
endangered northeastern bulrush, Scirpus ancistrochaetus Schuyler. Plant Ecology 139: 125-131.† pdf
7. Moran, P.J., and D.F. Cipollini. 1998. Effect of fungal infection and mechanical stress on peroxidase activity and resistance to pests in cucumber. Journal of Phytopathology 147: 313-316.
6. Cipollini, D.F. 1998. The induction of soluble peroxidase activity in leaves of bean plants by wind induced mechanical perturbation. American Journal of Botany 85: 1586-1591 pdf
5. Cipollini, D.F. 1998. Induced defenses and phenotypic plasticity. Trends in Ecology and Evolution 13: 200.
4. Cipollini, D.F. 1997. Wind-induced mechanical stimulation increases pest resistance in common bean. Oecologia 111: 84-90. pdf
3. Cipollini, D.F. 1997. Gibberellic acid treatment reduces the tolerance of field-grown common bean to leaf removal. Journal of Plant Growth Regulation 16: 123-127.
2. Cipollini, D.F. and J.C. Schultz. 1997. Wind and trees. Trends in Ecology and Evolution 12: 276-277.
Cipollini, D.F., S.J. Newell, and A.J. Nastase. 1994. The estimation of total carbohydrates in the
nectar of the pitcher plant, Sarracenia purpurea L. American
CURRENT LAB PEOPLE:
Deah Lieurance (E.S. Ph.D. Program 2008-present):† Exploring the enemy release hypothesis in invasive Lonicera species.
Samantha (Sam) Davis (E.S. Ph.D. Program, 2010-present):† Direct and indirect impacts of garlic mustard on a rare woodland butterfly.
Andy Officer (M.S. Program, 2009-present):† Interactions between garlic mustard, and mutualistic and pathogenic fungi.
Kelly Schradin (M.S. Program, 2010-present):† The role of plant-soil feedback in the invasiveness of Lonicera maackii.
Wang Qin (M.S. Biology 2010):† Variation in resistance of Asian and North American ash species to the Emerald Ash Borer.† Current: MBA program Wright State University
Barto (M.S. Biology 2003): Testing the
optimal defense theory and growth differentiation balance hypothesis in Arabidopsis
(Ph.D. Environmental Sciences 2008): An assessment of the allelopathic potential of Alliaria petiolata.† Current: Post-Doc at
Eusondia Barto †Undergraduate honorís thesis (2006):† Influence of wounding and emerald ash borer
extracts on defense protein expression in white and green ash.† Current: PhD program in Microbiology,
Victoria Ciola (M.S. Program 2007-2009):† Distribution and host specificity of a powdery mildew infecting garlic mustard.† Science Editor, McGraw-Hill
Dan Romanek (M.S. Program 2008-2009):† Population and biogeographic variation in allelopathic effects of Lonicera maackii.† Chemical Remediation Industry
Stephanie Enright (M.S. Biology 2006) Direct and indirect impacts of powdery mildew on Alliaria petiolata.† Current: Lab Coordinator, Wittenberg University
Hillstrom (M.S. Biology 2005)
Plasticity in growth, antioxidant chemistry, and jasmonic
acid induced defenses of Alliaria petiolata across nutrient and moisture gradients.
Current:† PhD program in Plant
Sara Weber (M.S. Biology 2005) Impacts of Alliaria petiolata invasion on the genetic diversity of Impatiens capensis. Current: Environmental Specialist, Ashland Chemical
Biology 2004) Impacts of honeysuckle on neighboring plant performance and
community dynamics.† Past: Research
Michelle Sipe (M.S. Biology 2004) Thesis title: Specificity in the induction and effects of chemical defenses in Brassica kaber Current:
Cherissa Rainey† Undergraduate research technician 2006-2008
Bill Gruner was an undergraduate technician in the lab during 2004-2005.† Current: Graduate of MS Program in Biological Sciences, Wright State, Researcher at WPAFB
Busch served as a research technician during the summer of 2000, who went
on to receive his PhD at
Current: faculty at
Neela Kumar was a M.S. student in the lab for
9 months during 2001.
Current: Graduate of PhD program in Botany at
Slemmons was an independent researcher and
undergraduate research technician from Fall
1999-Spring 2002, who went on to receive an MS in Environmental Sciences at
†Lori Healey was an undergraduate
research technician from Spring 2002 to Spring 2003,
who went on to complete her MS in Microbiology and Immunology at
If you are interested in learning more about our lab, please contact me by phone or e-mail.
Bio 444 Plant Physiology
ES 712 Genes, Organisms, and Ecosystems
Bio 800 Field Ecology Seminar
Hiking, camping, live music, sports ... and good beer
Revised: April 2011