The BMB department has modern biochemical and molecular biological research equipment, including visible-ultraviolet recording spectrophotometers, spectrofluorometers, DNA synthesizer, circular dichroism spectrophotometer, FPLC, Silicon Graphics molecular modeling system, DNA array, real-time PCR system, stopped-flow reaction analyzer, liquid scintillation counters, gamma counters, ultracentrifuges, various kinds of electrophoresis equipment, phosphorimager, gas-liquid chromatographs, high pressure liquid chromatographs, and tissue culture facilities. NMR, mass spectrometry, and biocontainment facilities are available for departmental use. A modern, well-equipped laboratory animal facility is also available.
Center for Genomics Research
The Center for Genomics Research (CGR) is a core research facility of the Boonshoft School of Medicine and the College of Science and Mathematics at Wright State University. CGR's primary mission is to provide support and core facilities for Wright State research faculty engaged in basic and clinical research in the areas of gene expression, flow cytometry and genotyping. The center strives to cultivate collaborations between basic and clinical researchers at Wright State through training of personnel, collaborative projects undertaken at CGR and joint grant submissions involving researchers and CGR members.
Center membership is open to all WSU-affiliated faculty. CGR charges investigator grants for use of certain equipment to offset the costs for contracts, reagents and staff time required for equipment maintenance. For groups not associated with WSU, the center works on genomic research projects on a fee-for-service basis. For more information about CGR membership and services, contact Dr. Michael Markey (phone: (937) 775-4536; email: firstname.lastname@example.org.)
Collaborate with CGR
What can CGR do for my project?
CGR has collaborated on projects at WSU and external to the university. CGR can help in the design of experiments, provide expertise and "hands" for performing experiments, perform data analysis and interpretation, and utilize facilities for quantitative PCR, flow cytometry, microarrays, and next generation sequencing. Study topics may include:
- Gene expression
- Chromosomal copy number changes
- Single nucleotide polymorphism genotyping
- Exon and splice junction usage
- DNA next generation sequencing
- Structural variants
- Point mutations
- Copy number variation
- Exome sequencing
- Targeted sequencing
- Whole genome sequencing
- RNA sequencing (RNA-seq)
- Gene expression
- Gene fusion
- Splice variants
- Transcriptome sequencing
- Targeted RNA-seq
- Chromatin immunoprecipitation sequencing (ChIP-seq)
- Methylation sequencing (Methyl-seq)
- Flow cytometry
- Cell cycle analysis
- Surface marker expression
- Other methods in genomics
When should I contact CGR?
CGR can be most effective if involved from the beginning of a project. CGR can consult during the grant writing process and provide letters of support. It is preferred that CGR be written into grant-funded research as a collaborator. For questions or begin the process, contact Michael Markey at (937) 775-4536.
|ABI 2700 thermocycler||PCR and other thermocycling|
|ABI 7900 HT SDS with PCR clean room||TaqMan based RT-PCR
Syber green based RT-PCR
|ABI GeneAmp 9700 thermocycler||PCR and other thermocycling|
|ABI QuantStudio 7 thermocycler||Real-time PCR and Array Cards|
|ABI StepOne thermocycler||Real-time PCR|
|Affymetrix GeneChip System||Gene expression profiling
Global human genotyping
|Agilent Bioanalyzer 2100||RNA, DNA and protein quantification|
|Beckman DU-50||UV-Vis spectrophotometry|
|Cepheid SmartCycler thermocycler||Gradient and real-time PCR|
|Eppendorf MasterCycler thermocycler||PCR and other thermocycling|
|Fuji LAS 4000||Captures images; chemiluminescence; ethidium bromide; white light images|
|IonTorrent PGM||Next-generation sequencing|
|Nanodrop spectrophotometer||RNA, DNA, and protein quantification|
|PerkinElmer Piezorray||Custom DNA and protein array creation|
|QIAcube||Automation of Qiagen kits|
|RNA clean area||Provides an RNase-free work area and tools for RNA extraction|
- Basic: The basic subscription includes use of the PCR setup room and standard thermocyclers, as well as the RNA prep room for $50/year per lab. This subscription counts toward the annual use fee for one other piece of equipment. For example, a basic subscription plus the Nanodrop would be $100.
- Cepheid SmartCycler Gradient and qPCR Thermocycler: Unlimited annual use of the Cepheid SmartCycler real-time thermocycler is $150; cuvettes are available for $0.60 each. The SmartCycler is designed for portability. Users wishing to borrow the SmartCylcer for field work should contact the CGR.
- Fuji LAS-4000 Imaging System: Unlimited annual use of the LAS-4000 is $150.
- Nanodrop Spectrophotometer: Unlimited annual use of the Nanodrop is $100. Pipettors and a variety of nucleic acid elution buffers are available for your convenience.
Per Use Fees
- Affymetrix GeneChip Microarrays: All users wishing to perform GeneChip experiments will pay for their own reagents and supplies, including GeneChips, which can be purchased through CGR. Additionally, CGR charges $100 per chip towards the expense of maintenance. WSU users pay only $50 per chip.
- Agilent 2100 Bioanalyzer: Bioanalyzer chips are paid for by users as needed and can be run for $10/chip by WSU users. Chips can be purchased through the CGR at cost for $30.
- Applied Biosystems 7900HT Sequence Detection System: There is a $12 per plate charge for use of the ABI real-time thermocycler. Users pay for their own TaqMan or SYBR Green RT-qPCR kits, as well as tips, plates, and other supplies.
- Accuri C6 Cytometer: Charges for the flow cytometer are still $30 per hour to cover sheath fluid and maintenance. Users should provide their own tubes and reagents.
It is the goal of the Center for Genomics Research to remain accessible to all WSU customers. Users who feel unable to afford subscription fees should contact Dr. Michael Markey at email@example.com.
Nuclear Magnetic Resonance Laboratory
The WSU Nuclear Magnetic Resonance (NMR) Laboratory houses two research instruments: (1) a 8.5 Tesla Wide-Bore (89 mm clear bore) 360 MHz NMR System equipped with a Tecmag Discovery Console; and (2) a Varian INOVA 600 NMR Spectrometer.
The 360 NMR system has various Bruker commercial probes for proton, fluorine, and broadband capabilities. This system is also used for NMR studies in vivo and is equipped with several home-built probes that can accommodate mice or rats.
The Varian INOVA 600 is equipped with:
- a triple resonance inverse probe (1H/13C/X),
- a broadband observe probe with a 13C/1H decouple channel (X/13C/1H),
- an Inverse Nanoprobe for 1H MAS spectroscopy in small volumes (40 ul),
- a variable temperature unit (FTS Systems, XR401 Air-Jet Crystal Cooler, -40 °C to +100 °C), and
- a Zymark XP Robotics Sample Changer (50 sample capacity).