Autoclave / Sterilizer Use
Autoclave is the process of sterilization by the use of heated steam under
pressure to kill vegetative microorganisms and directly exposed spores.
Common temperature and pressure for being effective is 121°C (250°F) at 15
psi (pounds per square inch) over pressure for 15 minutes.
Special cases may require a variation of the steam temperature and pressure used.
Not all materials are capable of being exposed to steam sterilization in an autoclave. For those items
that can not be steam sterilized, there are other alternatives in the form of gas sterilization or chemical
disinfectants that can be used given proper consideration to practicality, the desired level of disinfection
and potential hazards associated with handling of the item and the disinfectant.
For complete guidelines, please read APPENDIX E of this manual .
For information, contact Marjorie Markopoulos at (937) 775-2797.